FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL

(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170

FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL

PHYTOCHEMICAL SCREENING AND ISOLATION OF STEROLS FROM Cassytha filiformis LINN
Pages: 428-432
A. A. Ambi, M. B. Nasirudeen, A. T. Mora and F. G. Nuru


keywords: β-sitosterol, Cassytha filiformis, FT-IR, NMR, RumfarGada, stigmasterol

Abstract

Cassytha filiformis, a leafless and perennial vine with small scales as a replacement of the leaves is currently being used in the treatment of various disease conditions such as jaundice, fever and malaise. Preliminary phytochemical screening of various extracts (Petroleum ether, Ethyl acetate and methanol) showed that the whole plant contains alkaloids, steroidal nucleus, phenolic nucleus, terpenoids, flavonoids and saponins. Isolated compound 5B from petroleum ether extracts was analyzed using various test ranging from physical (Melting point 144-146oC uncorrected and Solubility in ethyl acetate and chloroform), chemical (Positive reaction using Libermann-Buchard test) and spectroscopic techniques (NMR, FT-IR and MS) in order to determine the identity of the isolated compound. Based on the result obtained from these analyses and comparing the data with that reported in the literatures, 5B contained β-sitosterol and stigmasterol.

References

Anjoo K & Ajay K Saluja 2011. Isolation of stigmasterol and βsitosterol from petroleum ether extract of aerial parts of Ageratum conyzoides (Asteraceae). Int. J. Pharm. & Pharm. Sci., 3(1): 94-96. Batta AK, Xuab G, Honda A, Miyazaki T & Salen G 2006. Stigmasterol reduces plasma cholesterol levels and inhibits hepatic synthesis and intestinal absorption in the rat. J. Pharml Sci., 55(3): 292-299. Burkill HM 1995. The Useful Plants of West and Tropical Africa (Vol. 3). Royal Botanical Garden Kew (UK), pp. 39-41. Chulet R, Joseph L, George M & Pradhan P 2010. Pharmacognostic standardization and phytochemical screening of Albizzialebbeck. J. Chem. Pharm. Res., 2(1): 432-443. EP 2011. European Pharmacopoeia. (7th Edition, supplement 1), Council of Europe, Strasbourg France, pp. 17-78. Jamal AK, Yaacob WA & Din LB 2009. A chemical study on Phyllanthuscolumnaris. Eur. J. Sci. Res., 28(1): 76‐81. Kametani T & Furuyama H 1987. Synthesis of vitamin D3 and related compounds. Med. Res. Rev., 7(2): 147–171. Khandelwal KR 2005. Practical Pharmacognosy, Techniques and Experiments. 13th ed. Pune, India: NiraliPrakashan, pp. 18-153. Mythili S, Gajalakshmi S, Sathiavelu A & Sridharan TB 2011. Pharmacological activities of Cassytha filiformis: A review. Asian J. Plant Sci. Res., 1(1): 77-83. Quetin-Leclercq J, Hoet S, Block S, Wautier MC & Stévigny C 2004. Studies on Cassytha filiformis from Benin: isolation, biological activities and quantification of aporphines. Proc. of Biores. Drug Disc. Devt., 88- 107. Pearson D 1991. The Chemical Analysis of Foods, 7th edition. Sakshy S, Hullatti KK, Prasanna SM &Paras S 2010. Comparative morphoanatomical and preliminary phytochemical studies of cuscutareflexa and Cassytha filiformis Linn. Int. J. Pharm. Pharm. Sci., 2(1): 59-64. Sharma S, Hullatti KK, Prasanna SM, Kuppast IJ & Sharma P 2009. Comparative Study of Cuscutareflexa and Cassytha filiformis for Diuretic Activity. Pharm. Res., 1: 327-330. Sundararaman P & Djerassi C 1977. A convenient synthesis of progesterone from stigmasterol. J. Org. Chem., 42(22): 3633–3634. Vipin KG & William RN 1984. Codisterol and other ∆s sterols in the seeds of Cucurbita maxima. Phytochem., 23(12): 925-2929. Watson L & Dallwitz MJ 1993. Lauraceae The Families of Flowering Plants Retrieved from: delta-intkey.com/angio/. 3rd October, 2016. WHO 2011. Quality control methods for herbal materials. WHO library cataloguing in publication.

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